Adaptation of Tobacco Cells to NaCl

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Solute Accumulation in Tobacco Cells Adapted to NaCl.

Cells of Nicotiana tabacum L. var Wisconsin 38 adapted to NaCl (up to 428 millimolar) which have undergone extensive osmotic adjustment accumulated Na(+) and Cl(-) as principal solutes for this adjustment. Although the intracellular concentrations of Na(+) and Cl(-) correlated well with the level of adaptation, these ions apparently did not contribute to the osmotic adjustment which occurred du...

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Stable NaCl Tolerance of Tobacco Cells Is Associated with Enhanced Accumulation of Osmotin.

Osmotin is a major protein which accumulates in tobacco cells (Nicotiana tabacum L. var Wisconsin 38) adapted to low water potentials. Quantitation of osmotin levels by immunoblots indicated that cells adapted to 428 millimolar NaCl contained 4 to 30 times the level of osmotin found in unadapted cells, depending on the stage of growth. Unadapted cells accumulated low levels of osmotin with appa...

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Carbon Use Efficiency and Cell Expansion of NaCl-Adapted Tobacco Cells.

Carbon use efficiencies (gram cell organic dry weight accumulated per gram sugar assimilated from the medium) of unadapted and NaCl-adapted (428 millimolar) cells of tobacco (Nicotiana tabacum L. var Wisconsin 38) were determined to evaluate metabolic costs associated with growth and survival in a saline environment. No net increase in carbon costs was associated with salt adaptation. At low su...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1985

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.79.1.118